Journal: Cell Biology International
Article Title: Distribution of Troy ( Tnfrsf19 ) in the Gastric Gland During Postnatal Development: Effects of Early Weaning
doi: 10.1002/cbin.70021
Figure Lengend Snippet: Effects of early weaning at Troy gene expression and protein levels in the stomach. (A) Illustrative diagram for experimental design. (B) Comparison of Tnfrsf (TROY) between the suckling (S) and early weaned (EW) groups at 18 and 60 pnd. (C) Representative immunoblots for Troy and the respective endogenous constitutive control used (β‐actin). Indicated are the groups: suckling (S) and subjected to early weaning (EW) at 18 and 60 pnd, samples were distributed on the same 12% SDS‐polyacrylamide gel for each age. The results were obtained from the integrated optical density (IOD) (UA) and compared to the β‐actin reading. The immunoblots were carried out in duplicates, and the bands above Troy are recurrent and appeared in duplicates, possibly due to the use of a polyclonal antibody. Each point represents an animal and the means ± SD are indicated. Results analyzed by Student's t ‐test with means and ± SD. * p < 0.05.
Article Snippet: The sections were incubated overnight at 4°C with rabbit polyclonal antibody against Troy (20 μg/mL, M‐220, Santa Cruz Biotechnology).
Techniques: Gene Expression, Comparison, Western Blot, Control